Concentration Assay:Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads.
Article Snippet: .. Moreover, the analysis indicated the significant impact of the concentration of urea, wheat flour and MgSO4.7H2O while the impact Table 3 MIC values Sample MIC value (mg/mL) Staphylococcus aureus ATCC 6538 Candida albicans ATCC 10231 D1 62.5 250 D2 62.5 500 D3 62.5 125 D4 31.25 500 Table 4 Fibrinolytic and anticoagulant activities Sample Fibrinolytic activity (%) Anticoagulant activity (Clotting time in sec) D1 15 ± 0.17 18 ± 0.09 D2 50 ± 0.25 33 ± 0.18 D3 60 ± 0.06 145 ± 0.06 D4 40 ± 0.09 91 ± 0.04 Standard (Hemoclar) 40 – Standard (Heparin) – 90 Table 5 Plackett–Burman design Run number Sucrose concentration (%) pH Urea concentration (%) Wheat flour concentration (%) K2HPO4 concentration (%) MgSO4.7H2O concentration (%) ZnSO4 concentration (%) Enzyme activity (U/mL) 1 15 (−) 7.5 (−) 0.04 (−) 10 (+) 0.5 (+) 0.015 (+) 0.0025 (−) 40.683 2 20 (+) 7.5 (−) 0.04 (−) 6 (−) 0.3 (−) 0.015 (+) 0.0075 (+) 36.973 3 15 (−) 9.5 (+) 0.04 (−) 6 (−) 0.5 (+) 0.005 (−) 0.0075 (+) 30.171 4 20 (+) 9.5 (+) 0.04 (−) 10 (+) 0.3 (−) 0.005 (−) 0.0025 (−) 38.008 5 15 (−) 7.5 (−) 0.08 (+) 10 (+) 0.3 (−) 0.005 (−) 0.0075 (+) 34.254 6 20 (+) 7.5 (−) 0.08 (+) 6 (−) 0.5 (+) 0.005 (−) 0.0025 (−) 31.044 7 15 (−) 9.5 (+) 0.08 (+) 6 (−) 0.3 (−) 0.015 (+) 0.0025 (−) 33.366 8 20 (+) 9.5 (+) 0.08 (+) 10 (+) 0.5 (+) 0.015 (+) 0.0075 (+) 33.263 Fig. 2 Antimicrobial activity of the precipitated exo-polysaccharide samples against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231 in which 1, 2, 3 and 4 were the precipitated dextran’s of molecular weight 51, 75, 100 and 168 KDa, respectively 1 3 Page 7 of 18 16 3 Biotech (2026) 16:16 analysis of the results indicated that the R2 value for the applied design was 0.9624 and the F-value was 14.24 with P-value of 0.005. ..
Activity Assay:Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads.
Article Snippet: .. Moreover, the analysis indicated the significant impact of the concentration of urea, wheat flour and MgSO4.7H2O while the impact Table 3 MIC values Sample MIC value (mg/mL) Staphylococcus aureus ATCC 6538 Candida albicans ATCC 10231 D1 62.5 250 D2 62.5 500 D3 62.5 125 D4 31.25 500 Table 4 Fibrinolytic and anticoagulant activities Sample Fibrinolytic activity (%) Anticoagulant activity (Clotting time in sec) D1 15 ± 0.17 18 ± 0.09 D2 50 ± 0.25 33 ± 0.18 D3 60 ± 0.06 145 ± 0.06 D4 40 ± 0.09 91 ± 0.04 Standard (Hemoclar) 40 – Standard (Heparin) – 90 Table 5 Plackett–Burman design Run number Sucrose concentration (%) pH Urea concentration (%) Wheat flour concentration (%) K2HPO4 concentration (%) MgSO4.7H2O concentration (%) ZnSO4 concentration (%) Enzyme activity (U/mL) 1 15 (−) 7.5 (−) 0.04 (−) 10 (+) 0.5 (+) 0.015 (+) 0.0025 (−) 40.683 2 20 (+) 7.5 (−) 0.04 (−) 6 (−) 0.3 (−) 0.015 (+) 0.0075 (+) 36.973 3 15 (−) 9.5 (+) 0.04 (−) 6 (−) 0.5 (+) 0.005 (−) 0.0075 (+) 30.171 4 20 (+) 9.5 (+) 0.04 (−) 10 (+) 0.3 (−) 0.005 (−) 0.0025 (−) 38.008 5 15 (−) 7.5 (−) 0.08 (+) 10 (+) 0.3 (−) 0.005 (−) 0.0075 (+) 34.254 6 20 (+) 7.5 (−) 0.08 (+) 6 (−) 0.5 (+) 0.005 (−) 0.0025 (−) 31.044 7 15 (−) 9.5 (+) 0.08 (+) 6 (−) 0.3 (−) 0.015 (+) 0.0025 (−) 33.366 8 20 (+) 9.5 (+) 0.08 (+) 10 (+) 0.5 (+) 0.015 (+) 0.0075 (+) 33.263 Fig. 2 Antimicrobial activity of the precipitated exo-polysaccharide samples against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231 in which 1, 2, 3 and 4 were the precipitated dextran’s of molecular weight 51, 75, 100 and 168 KDa, respectively 1 3 Page 7 of 18 16 3 Biotech (2026) 16:16 analysis of the results indicated that the R2 value for the applied design was 0.9624 and the F-value was 14.24 with P-value of 0.005. ..
Coagulation:Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads.
Article Snippet: .. Moreover, the analysis indicated the significant impact of the concentration of urea, wheat flour and MgSO4.7H2O while the impact Table 3 MIC values Sample MIC value (mg/mL) Staphylococcus aureus ATCC 6538 Candida albicans ATCC 10231 D1 62.5 250 D2 62.5 500 D3 62.5 125 D4 31.25 500 Table 4 Fibrinolytic and anticoagulant activities Sample Fibrinolytic activity (%) Anticoagulant activity (Clotting time in sec) D1 15 ± 0.17 18 ± 0.09 D2 50 ± 0.25 33 ± 0.18 D3 60 ± 0.06 145 ± 0.06 D4 40 ± 0.09 91 ± 0.04 Standard (Hemoclar) 40 – Standard (Heparin) – 90 Table 5 Plackett–Burman design Run number Sucrose concentration (%) pH Urea concentration (%) Wheat flour concentration (%) K2HPO4 concentration (%) MgSO4.7H2O concentration (%) ZnSO4 concentration (%) Enzyme activity (U/mL) 1 15 (−) 7.5 (−) 0.04 (−) 10 (+) 0.5 (+) 0.015 (+) 0.0025 (−) 40.683 2 20 (+) 7.5 (−) 0.04 (−) 6 (−) 0.3 (−) 0.015 (+) 0.0075 (+) 36.973 3 15 (−) 9.5 (+) 0.04 (−) 6 (−) 0.5 (+) 0.005 (−) 0.0075 (+) 30.171 4 20 (+) 9.5 (+) 0.04 (−) 10 (+) 0.3 (−) 0.005 (−) 0.0025 (−) 38.008 5 15 (−) 7.5 (−) 0.08 (+) 10 (+) 0.3 (−) 0.005 (−) 0.0075 (+) 34.254 6 20 (+) 7.5 (−) 0.08 (+) 6 (−) 0.5 (+) 0.005 (−) 0.0025 (−) 31.044 7 15 (−) 9.5 (+) 0.08 (+) 6 (−) 0.3 (−) 0.015 (+) 0.0025 (−) 33.366 8 20 (+) 9.5 (+) 0.08 (+) 10 (+) 0.5 (+) 0.015 (+) 0.0075 (+) 33.263 Fig. 2 Antimicrobial activity of the precipitated exo-polysaccharide samples against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231 in which 1, 2, 3 and 4 were the precipitated dextran’s of molecular weight 51, 75, 100 and 168 KDa, respectively 1 3 Page 7 of 18 16 3 Biotech (2026) 16:16 analysis of the results indicated that the R2 value for the applied design was 0.9624 and the F-value was 14.24 with P-value of 0.005. ..
Molecular Weight:Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads.
Article Snippet: .. Moreover, the analysis indicated the significant impact of the concentration of urea, wheat flour and MgSO4.7H2O while the impact Table 3 MIC values Sample MIC value (mg/mL) Staphylococcus aureus ATCC 6538 Candida albicans ATCC 10231 D1 62.5 250 D2 62.5 500 D3 62.5 125 D4 31.25 500 Table 4 Fibrinolytic and anticoagulant activities Sample Fibrinolytic activity (%) Anticoagulant activity (Clotting time in sec) D1 15 ± 0.17 18 ± 0.09 D2 50 ± 0.25 33 ± 0.18 D3 60 ± 0.06 145 ± 0.06 D4 40 ± 0.09 91 ± 0.04 Standard (Hemoclar) 40 – Standard (Heparin) – 90 Table 5 Plackett–Burman design Run number Sucrose concentration (%) pH Urea concentration (%) Wheat flour concentration (%) K2HPO4 concentration (%) MgSO4.7H2O concentration (%) ZnSO4 concentration (%) Enzyme activity (U/mL) 1 15 (−) 7.5 (−) 0.04 (−) 10 (+) 0.5 (+) 0.015 (+) 0.0025 (−) 40.683 2 20 (+) 7.5 (−) 0.04 (−) 6 (−) 0.3 (−) 0.015 (+) 0.0075 (+) 36.973 3 15 (−) 9.5 (+) 0.04 (−) 6 (−) 0.5 (+) 0.005 (−) 0.0075 (+) 30.171 4 20 (+) 9.5 (+) 0.04 (−) 10 (+) 0.3 (−) 0.005 (−) 0.0025 (−) 38.008 5 15 (−) 7.5 (−) 0.08 (+) 10 (+) 0.3 (−) 0.005 (−) 0.0075 (+) 34.254 6 20 (+) 7.5 (−) 0.08 (+) 6 (−) 0.5 (+) 0.005 (−) 0.0025 (−) 31.044 7 15 (−) 9.5 (+) 0.08 (+) 6 (−) 0.3 (−) 0.015 (+) 0.0025 (−) 33.366 8 20 (+) 9.5 (+) 0.08 (+) 10 (+) 0.5 (+) 0.015 (+) 0.0075 (+) 33.263 Fig. 2 Antimicrobial activity of the precipitated exo-polysaccharide samples against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231 in which 1, 2, 3 and 4 were the precipitated dextran’s of molecular weight 51, 75, 100 and 168 KDa, respectively 1 3 Page 7 of 18 16 3 Biotech (2026) 16:16 analysis of the results indicated that the R2 value for the applied design was 0.9624 and the F-value was 14.24 with P-value of 0.005. ..
Significance Assay:Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads.
Article Snippet: .. Moreover, the analysis indicated the significant impact of the concentration of urea, wheat flour and MgSO4.7H2O while the impact Table 3 MIC values Sample MIC value (mg/mL) Staphylococcus aureus ATCC 6538 Candida albicans ATCC 10231 D1 62.5 250 D2 62.5 500 D3 62.5 125 D4 31.25 500 Table 4 Fibrinolytic and anticoagulant activities Sample Fibrinolytic activity (%) Anticoagulant activity (Clotting time in sec) D1 15 ± 0.17 18 ± 0.09 D2 50 ± 0.25 33 ± 0.18 D3 60 ± 0.06 145 ± 0.06 D4 40 ± 0.09 91 ± 0.04 Standard (Hemoclar) 40 – Standard (Heparin) – 90 Table 5 Plackett–Burman design Run number Sucrose concentration (%) pH Urea concentration (%) Wheat flour concentration (%) K2HPO4 concentration (%) MgSO4.7H2O concentration (%) ZnSO4 concentration (%) Enzyme activity (U/mL) 1 15 (−) 7.5 (−) 0.04 (−) 10 (+) 0.5 (+) 0.015 (+) 0.0025 (−) 40.683 2 20 (+) 7.5 (−) 0.04 (−) 6 (−) 0.3 (−) 0.015 (+) 0.0075 (+) 36.973 3 15 (−) 9.5 (+) 0.04 (−) 6 (−) 0.5 (+) 0.005 (−) 0.0075 (+) 30.171 4 20 (+) 9.5 (+) 0.04 (−) 10 (+) 0.3 (−) 0.005 (−) 0.0025 (−) 38.008 5 15 (−) 7.5 (−) 0.08 (+) 10 (+) 0.3 (−) 0.005 (−) 0.0075 (+) 34.254 6 20 (+) 7.5 (−) 0.08 (+) 6 (−) 0.5 (+) 0.005 (−) 0.0025 (−) 31.044 7 15 (−) 9.5 (+) 0.08 (+) 6 (−) 0.3 (−) 0.015 (+) 0.0025 (−) 33.366 8 20 (+) 9.5 (+) 0.08 (+) 10 (+) 0.5 (+) 0.015 (+) 0.0075 (+) 33.263 Fig. 2 Antimicrobial activity of the precipitated exo-polysaccharide samples against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231 in which 1, 2, 3 and 4 were the precipitated dextran’s of molecular weight 51, 75, 100 and 168 KDa, respectively 1 3 Page 7 of 18 16 3 Biotech (2026) 16:16 analysis of the results indicated that the R2 value for the applied design was 0.9624 and the F-value was 14.24 with P-value of 0.005. ..
|